Journal: Cells

Article Title: WWOX Induction Promotes Bcl-X L and Mcl-1 Degradation Through a Lysosomal Pathway upon Stress Responses

doi: 10.3390/cells15030270

Figure Lengend Snippet: WWOX promotes Bcl-X L and Mcl-1 protein downregulation through a lysosomal degradation pathway. ( A ) Wwox +/+ MEFs were treated with serum-free medium for 36 h with or without the presence of a proteasome inhibitor, MG132 (1 μM), during the last 18 h of serum starvation. ( B ) Wwox +/+ MEFs were treated with serum-free medium for 32 h with or without the presence of NH 4 Cl (25 mM) or a lysosome inhibitor, chloroquine (CQ; 100 μM), during the last 4 and 8 h of serum starvation. ( C ) Wwox +/+ MEFs were treated with serum-free medium for 32 h with or without the presence of a lysosomal enzyme inhibitor E64d (30 μM) or pepstatin A (15 μM) during the last 4 and 8 h of serum starvation. Bcl-X L , Mcl-1, and WWOX protein expression was examined by Western blotting. β-actin was used as a loading control. Inhibition of Itch protein degradation in cycloheximide (CHX)-treated cells was used as a MG132 reagent control. The representative results from four repeated experiments are shown. Densitometric analysis for quantification of protein levels is shown in .

Article Snippet: Pepstatin A was obtained from Tocris (Bristol, UK).

Techniques: Expressing, Western Blot, Control, Inhibition